The asialoglycoprotein receptor clears glycoconjugates terminating with sialic acid 2,6GalNAc

The asialoglycoprotein receptor clears glycoconjugates terminating with sialic acid 2,6GalNAc

Endogenous ligands have not, to date, been identified for the asialoglycoprotein receptor (ASGP-R), which is abundantly expressed by parenchymal cells in the liver of mammals. On the basis of the rapid clearance of BSA bearing multiple chemically coupled sialic acid (Sia) 2,6GalNAc 1,4GlcNAc 1,2Man tetrasaccharides (SiaGGnM-BSA) from the circulation, and the ability of the ASGP-R hepatic lectin...

Terminal Sialic Acid of Glycoconjugates on the Surface of Intestinal Epithelial Cells

Helicobacter pylori and neutrophils: sialic acid-dependent binding to various isolated glycoconjugates.

Helicobacter pylori has been shown to agglutinate erythrocytes in a sialic acid-dependent manner. However, very few studies have examined relevant target cells in the human stomach. Neutrophils are required for the onset of gastritis, and the inflammatory reaction may be induced on contact between bacteria and neutrophils. In the present work, glycolipids and glycoproteins were isolated from ne...

Enterovirus 70 binds to different glycoconjugates containing alpha2,3-linked sialic acid on different cell lines.

Enterovirus 70 (EV70), the causative agent of acute hemorrhagic conjunctivitis, exhibits a restricted tropism for conjunctival and corneal cells in vivo but infects a wide spectrum of mammalian cells in culture. Previously, we demonstrated that human CD55 is a receptor for EV70 on HeLa cells but that EV70 also binds to sialic acid-containing receptors on a variety of other human cell lines. Vir...

The role of sialic acid synthases in sialic acid biosynthesis

Introduction The sialic acids comprise a diverse family of nine-carbon keto-sugars, the majority of which are structural derivatives of neuraminic acid (Fig. 1). Neuraminic acid has an amino moiety at the C-5 position, and carboxylate at C-2 which confers negative charge at physiological pH. Whereas unsubstituted neuraminic acid is not known to exist in nature, over 40 substituted variations (c...